In vitro regeneration of some selected mustard genotypes (Brassica spp )

In vitro regeneration of some selected mustard genotypes (Brassica spp )

Bangladesh J. Environ. Sci., Vol. 36, 47-50, 2019 @ BAED ISSN 1561-9206

IN VITRO REGENERATION OF SOME SELECTED MUSTARD GENOTYPES (Brassica spp )

N. Sultana1, I. Sultana2 and S. Sultana3

1Dept of Genetics and Plant Breeding, Patuakhali Science and Technology University, Patuakhali

2Agriculture Department, Ghior Govt. College, Manikgonj;

3Lal Teer Seed Ltd. Gazipur

ABSTRACT

An experiment was conducted to find out in vitro performance of some mustard genotypes (Safal, Sampad and Tori-7). For germination, MS medium was used without any growth regulators. For callus induction, 2, 4-D was used @ 1.5 mg/L concentration and for regeneration, Indole Acetic Acid (IAA) as well as Benzyl Amino Purine (BAP) were used @ 0.5 mg/L and 1.0 mg/L, respectively. The Plant Growth Regulators were used as a single dose combination to ascertain only the genotypic differences. In genotypic sense, Tori-7 and Safal showed better performances compared to Sampad (92.73% and 94% callus induction for Tori-7 and Safal, respectively). On the other hand, genotypes Tori-7 and Sampad ensured better regeneration (72% for Tori-7 and 65% for Sampad) than that of Safal (50%). Therefore; Tori-7 may be termed as the best genotype in terms of both callus induction and regeneration. No statistically significant differences were found for this regarding days required for callus induction but in case of days required for regeneration, the genotypes varied significantly. Both the genotypes Safal (38.98 days) and Sampad (41.79 days) are early regenerating than Tori-7 (45.21 days). All of the genotypes under study have shown their suitability in tissue culture studies with varying abilities.

Key words: In vitro, regeneration, mustard genotypes.

Performance evaluation of cucumber genotypes grown in Narsingdi region of Bangladesh

Performance evaluation of cucumber genotypes grown in Narsingdi region of Bangladesh

Bangladesh J. Environ. Sci., Vol. 36, 63-66, 2019 @ BAED ISSN 1561-9206

PERFORMANCE EVALUATION OF CUCUMBER GENOTYPES GROWN IN NARSINGDI REGION OF BANGLADESH

R. Akter1, M. M. Rahman2, M. Moniruzzaman3, M. Salim4 and M. A. Alam5

1&2Regional Horticulture Research Station, Bangladesh Agricultural Research Institute, Shibpur, Narsingdi

3Agriculture Research Station, Bangladesh Agricultural Research Institute, Pahartali, Chittagong

4Tuber Crops Research Sub-center, Bangladesh Agricultural Research Institute, Munshiganj

5Bangladesh Sugarcrop Research Institute, Chunarughat sub-station, Hobiganj

(Corresponding authors’ e-mail: ruby8997@yahoo.com)

ABSTRACT
An experiment was conducted at the experimental field of Regional Horticulture Research Station, BARI, Shibpur, Narsingdi during March to May 2018 in a randomized complete block design. Eighteen genotypes of cucumber were investigated to evaluate their performance. Minimum days (30 days) required to male flower initiate in CS Nar 007 and CS Nar 008 and minimum days (33 days) required to female flower initiate in CS Nar 002 genotypes. The highest fruit length (30.38 cm) was found from CS Nar 004. On the other hand, the highest fruit width (10.30 cm) and weight (699.0 g) were found from CS Nar 005. Maximum number (7.00) of fruits plant-1 was rerecorded from CS Nar 004. The highest yield ha-1 (37.08 t) detected from CS Nar 004. Considering fruit size, number of fruits plant -1 and yield ha -1 the genotypes CS Nar 004 were showed better performance and may be recommended for cucumber production at Narsingdi region. Key words: Cucumber, genotypes, evaluation, fruit.