The success of Agrobacterium-mediated genetic transformation in deshi jute plants (Corchorus capsularis L.) depends on the selection of transformants. In the present research, the hygromycin sensitivity test was performed on wild-type seeds to obtain the most effective hygromycin concentration for selection of transgenic. Based on root growth, the most effective concentration of hygromycin was determined 25 mg/l. In this concentration of hygromycin, non-transgenic plants indicated no root elongation, as compared to transgenic plants. A clear decrease of root growth was optimized for the selection of transformants. Thus, for effective optimization and selection of transformants, we can make easy the laborious screening tasks in in planta transformation for further verification through polymerage chain reaction (PCR).
Key words: Hygromycin, selective marker, Agrobacterium tumefaciens, deshi jute.
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