ABSTRACT

A Petri dish and hydroponic culture experiment was conducted at Plant Physiology Laboratory, Department
of Crop Botany, Bangladesh Agricultural University, Mymensingh during the period from November 2017 to
April 2018 to investigate the effect of boron and aluminium and their interactions on seed germination and
seedling growth on wheat seedlings. The experiment comprised of two levels of B (0, 40 µM) and Al (0,
200 µM). The treatments combination were 0 µM B + 0 µM Al (control), 0 µM Al + 40 µM B, 200 µM Al +
40 µM B and 0 µM B + 200 µM Al and five wheat varieties viz; BARI GOM 23, BARI GOM 24, BARI
GOM 28, BARI GOM 27 and BARI GOM 30. The experiment was laid out in two factors Completely
Randomized Design with three replications. Application of 0 µM Al + 40 µM B had a higher radicle and
plumule length, germination percentages, root length, shoot length, leaf length, leaf sheath length, and fresh
and dry mass production in wheat. Results indicated that germination percentage, radicle and plumule length,
root and shoot length, leaf length, leaf sheath length, fresh and dry mass plant-1 were greater in boron
treatment but without aluminium. Application of 0 µM B + 200 µM Al resulted germination percentage,
radicle and plumule length, root and shoot length, leaf length, leaf sheath length, fresh and dry mass plant-1
were lowest in compare to other treatment. However, Aluminium had profound negative effect on
germination percentage, growth and developments of wheat seedlings but boron can ameliorate the
aluminium toxicity in every stage of growth and developments of wheat seedlings. Among the varieties,
BARI GOM 28 had highest tolerance to aluminium toxicity and positive boron response in respect of growth
and development.
Key words: Boron, aluminium, seed germination, seedling growth.
Introduction
The wheat crop is mainly cultivated under rain fed conditions where precipitation is less than 900 mm
annually. Wheat is grown both as spring and winter crop. It is widely grown throughout the temperate
zones (in Northern Europe up to 60° N) and in some tropical/sub-tropical areas at higher elevations. The
major centers are: Europe (131 million t grain, 27 million ha), the former USSR (108 million t grain, 48
million ha), North America (106 million t grain, 42 million ha), China (96 million t grain, 30 million ha)
and India (50 million t grain, 23 million ha). Aluminium stress associated with low soil pH affected soils
and there are more than one million ha of land with low pH in Bangladesh. On world-wide basis there are
nearly 2.6 billion ha of strongly acid soils with Al3+ toxicity (Dudal, 1976). Acid soil, by increasing Al3+
solubility increases its concentration at the rhizosphere. Al3+ toxicity inhibits plant growth by interfering
with the regulatory process of root growth and development (Foy and Taylor, 1998). To overcome the
situation Al3+ tolerant wheat germplasm may be helpful for the expansion of its cultivation in the areas of
acid soil. In Bangladesh, the tolerance grade of the existing gene pool of wheat against Al3+ toxicity is yet
to be determined .Thus, the present work was undertaken to determine the tolerance efficiency of some
high yielding varieties of wheat against different levels of Al3+ stress with respect to seed germination, root
and shoot growth, and dry matter yield at seedling stage. Boron (B) is an essential element for plant growth.
Boron has been referred to as one of the apoplastic elements mainly because it is localized in cell walls.
However, the real function of B in plant nutrition has not been completely elucidated. Wheat cultivars
differ markedly in their sensitivity to B deficiency Boron efficient cultivars of wheat displayed greater
ability to absorb B than B-inefficient cultivars when grown under a similar B supply (Subedi et al. 1999).