Potential uses of antifreeze proteins: a review

ABSTRACT

The freeze-tolerant organisms are gifted with the AFPs/THPs (antifreeze proteins/thermal hysteresis proteins), which have evolved as an adaptation in extreme cold conditions. AFPs have been identified in vertebrates, invertebrates, plants, bacteria, and fungi. In this review, we deal with, diverse applications in the fields of industry, medical and agriculture such as food technology, cell lines and organ preservation, transplantation and transfusion. AFPs have potential applications in agriculture for the production of economically valuable fishes against low temperature. Other proposed applications of AFPs are found in cryosurgery of tumors and as a component of ice-cream to prevent the formation of hard and large ice crystals. Furthermore, the present study focuses the new insights into the development of methodology to allow the production of synthetic AFPs as well as their potential uses.

Key words: Antifreeze protein, cryopreservation, food safety, cryosurgery.

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Computational tools for sequence based identification and Characterization of antifreeze proteins

ABSTRACT

Living organisms including fishes, microbes, and animals can live in extremely cold weather. To stay alive in cold environments, these species generate antifreeze proteins (AFPs), also referred to as ice binding proteins. Moreover, AFPs are extensively utilized in many important fields including medical, agricultural, industrial, and biotechnological. Several predictors were constructed to identify AFPs. However, due to the sequence and structural heterogeneity of AFPs, correct identification is still a challenging task. In this study, deals with several bio-informatical or computational tools which have been proposed for prediction of AFPs more precisely can predict AFPs more accurately and can participate in a significant role in medical, agricultural, industrial, and biotechnological fields.

Key words: Antifreeze protein, bioinformatics, physicochemical properties, homology modeling

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Characterization, structure-function relationship and mechanism of antifreeze proteins

ABSTRACT

Antifreeze proteins (AFPs) protect organisms from freezing and shows great diversity in structure and they have been found in a variety of organisms including vertebrates, invertebrates, plants, bacteria, and fungi. Study of AFPs is a promising issue due to diverse applications in the fields of industry, medical and agriculture such as food technology, cell lines and organ preservation, transplantation and transfusion. In this review, physicochemical, functional and structural features of some antifreeze proteins of each organism like insects, fish, plant, bacteria and fungi have been described. This review also focused on the structure-function relationship of antifreeze proteins. The result of structure analysis infers that AFPs are mostly hydrophobic and plays the important role in ice binding. In this study, recent new insights into the mechanism of action of four types of AFPs have also been discussed.

Key words: Antifreeze proteins, crystallization, mechanism, structure analysis.

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Effect of sowing time on leaf yield and yield attributes of jute varieties as vegetables

ABSTRACT

An experiment was conducted at Jute Agriculture Experimental Station, Manikganj to find out the leaf yield of different jute vegetable varieties at different sowing times. BJRI released three jute vegetable varieties such as BJRI deshi pat shak-1 (V1); BJRI deshi pat shak-1 (V2); BJRI deshi pat shak-1 (V3) and three sowing time i.e. 1st week of March (S1); 1st week of May (S2); 1st week of July (V3) were used as experimental treatments. The experiment was designed RCBD with three replicates. Results revealed that, the highest plant population, plant height, leaf number were found better in BJRI deshi pat shak-3 (Merha green), whereas leaf area, leaf fresh weight per plant and leaf yield were found maximum from BJRI deshi pat shak-1. On the other hand, out of three seasons March is more suitable for leaf yield.

Key words: Jute, leafy vegetable, leaf area, yield

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Assessment of kenaf seed qualities influenced by different storage container

ABSTRACT

Kenaf seeds lose their ability to germinate during storage, leading to a lack of quality seeds compared to planting requirements, hindering the expansion of kenaf cultivation in Bangladesh. The effects of different storage containers on the germination, electrical conductivity, vigor index and accelerated aging of kenaf seeds of three varieties were studied during a 6-month storage period from 20th February 2020 to 20th August 2020 in a room. Four different containers viz. C1= aluminum foil, C2= plastic pot, C3= earthen pot and C4= gunny bag was used to store kenaf seeds. Three varieties, V1=HC-95, V2=HC-2 and V3=BJRI Kenaf-3 were used in this experiment. This experiment revealed that the percentage of germination, vigor index and accelerated aging of all three varieties were reduced with increasing the storage time. On the other hand, electrical conductivity percent of all varieties were increased with increasing the storage period. Among the four containers it was observed that after 180 days of storage germination percentage, vigor index percent and accelerated aging percent were maximum for aluminum foil and the lowest were found in gunny bag whereas plastic pot and earthen pot remained in the middle position. On the other hand, at 180 days after storage the highest electrical conductivity was found in gunny bag (65.22%) and the lowest was found (39.89%) in aluminum foil. Germination rate at 180 DAS was recorded maximum in HC-95 (65.2%) and minimum in BJRI Kenaf-3 (57.3%). The highest electrical conductivity at 180 DAS was found in BJRI Kenaf-3 (42.78%) and the lowest was found in HC-2 (36.46%). The highest vigor index and accelerated aging was found (39.55% and 59.89%), respectively at 180 DAS were obtained in HC-95 (15.8%) and the lowest was found (34.24% and 56.88%, respectively) in BJRI Kenaf-3 at 180 DAS.  The results reveal that the quality of kenaf seeds decrease with increase the storage time.

Key words: Kenaf, seed quality, vigor, germination

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