Microbes having pectinolytic and xylanolytic without cellulolytic activity, are considered efficient retting microbes. Pectin of jute and mesta is highly methyl esterified and its degradation requires the combined action of a group of pectin degrading enzymes, poly-galacturonase and pectin/pectate lyase. Pectate lyase gene, pel-1 from pectinolytic bacteria has potentiality to break the pectin-based compound of jute that is the key function required during retting process. Pectinolytic bacteria harbor this gene naturally and ensure the lysis of pectin. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated protein (Cas) systems have revolutionized genome editing and greatly promoted the development of biotechnology. Modification and/or transcriptional activation of pectate lyase gene Pel1 by CRISPR/Cas9 to increase accumulation of pectin degrading enzymes, leading to initiate the pectin degradation activity and thereby contribute to their specialized pectinolytic function during the retting process. The variations in the pectate lyase genes possibly contribute to their specialized pectinolytic function during the retting process. In this study, our aim is to improve pectinolytic efficiency of bacteria through genome editing (CRISPR/Cas9) of Pectate lyase gene (Pel-1).
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