Chilli Pepper is the most important vegetable and widely consumed spice around the world. For genetic transformation or genome editing it is very important and prerequisite to establish an in vitro regeneration protocol. Callus formation is the essential step for regeneration. Pepper is highly recalcitrant plant. The present study was conducted to observe the high-quality callus induction from the leaf explant of hot pepper cultivar BARI Morich-2 under different hormonal concentration of auxin and cytokinin. Leaf from 10 -15 days grown in vitro grown seedlings was cultured on MS medium supplemented with 2,4-D (1.5 mg/l) and different combinations of BAP (0.2 mg/l, 0.4 mg/l and 0.6mg/l) or Zeatin (0.2 mg/l, 0.4 mg/l and 0.6mg/l). All combinations of phytohormones showed callus induction with variations of appearance and frequency.The leaf explants cultured on MS medium with 30% sucrose and 0.6 mg/l BAP + 1 .5mg/l 2,4-D were produced friable greenish calli and healthy protoplast from the calli were successfully isolated. Our findings speculated that leaf grown calli can be subjected to regenerate whole plant, genetic transformation, transient expression of gene of interest and application of genome editing tools.
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Microbes having pectinolytic and xylanolytic without cellulolytic activity, are considered efficient retting microbes. Pectin of jute and mesta is highly methyl esterified and its degradation requires the combined action of a group of pectin degrading enzymes, poly-galacturonase and pectin/pectate lyase. Pectate lyase gene, pel-1 from pectinolytic bacteria has potentiality to break the pectin-based compound of jute that is the key function required during retting process. Pectinolytic bacteria harbor this gene naturally and ensure the lysis of pectin. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated protein (Cas) systems have revolutionized genome editing and greatly promoted the development of biotechnology. Modification and/or transcriptional activation of pectate lyase gene Pel1 by CRISPR/Cas9 to increase accumulation of pectin degrading enzymes, leading to initiate the pectin degradation activity and thereby contribute to their specialized pectinolytic function during the retting process. The variations in the pectate lyase genes possibly contribute to their specialized pectinolytic function during the retting process. In this study, our aim is to improve pectinolytic efficiency of bacteria through genome editing (CRISPR/Cas9) of Pectate lyase gene (Pel-1).
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An experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh during the period from June to December 2017 to study the tolerance of local, inbred and hybrid rice varieties to weed stress in early stage of growth during aman season. The experiment consisted of six cultivars viz. Parsoom, Razamohon, BRRI dhan49, Binadhan-7, Agrodhan-13 and Dhani Gold and six different weeding regimes such as weedy (no weed control measure), weed free (weeds are not allowed throughout the growing season), weedy up to 21 DAT followed by (fb) weed free, weedy up to 28 DAT fb weed free, weedy up to 35 DAT fb weed free and weedy up to 42 DAT fb weed free. The experiment was laid out in a Randomized Complete Block Design (RCBD) with three replicates. Varieties were found to have significant effect on different crop characters and rice yield. The highest grain yield (6.437 t ha-1) as well as the straw yield (7.736 t ha-1) were found in BRRI dhan49 while the lowest grain yield (1.599 t ha-1) was obtained from Parsoom and the lowest straw yield (4.102 t ha-1) was found in Razamohon. The different growth, yield and yield contributing characters varied significantly in response to variety and different weeding regimes. The highest plant height (122.3 cm), highest number of total tillers hill-1 (7.476) and the highest number of effective tillers hill-1 (6.356) was produced in weeding treatment, weedy up to 28 DAT fb weed free. On the other hand, the shortest plant height (117.1 cm), the lowest number of total tillers hill-1 (6.919), the lowest number of effective tillers hill-1 (5.844) and the smallest panicle length (22.29 cm) were observed in weeding treatment, weedy up to 42 DAT fb weed free. So, in order to get efficient weeding regimes in response to higher grain yield in transplanted aman rice, BRRI dhan49 with weedy up to 21 DAT fb weed free treatment can be recommended.
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LTPs are induced during a wide range of biotic or abiotic stress. Very few LTPs have been shown to act through plasma membrane receptors or to be involved in the hypersensitive response (HR). AZI1, a lipid transfer protein (LTP) interacts with MPK3 to form protein complexes and thereby plays a pivotal role in resistance to abiotic stresses in planta. However, the particular functions of AZI1 orthologs in cereal species under biotic and abiotic stresses yet not been comprehensively defined. In this study, we isolate a novel wheat ortholog of the AZI1 gene, TaAZI1, from ACI gom-1 a popular wheat variety and functionally characterize. Sequence analysis indicated that TaAZI1 contains a hydrophobic protein from soybean (HPS)-like subfamily and has 61% identity with AZI1 proteins from Arabidopsis. Transcription of TaAZI1 was detected mostly in wheat stems and leaves.
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The experiment was conducted at the net house of Agro-environmental Chemistry Laboratory of the Department of Agricultural Chemistry of Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, Bangladesh during the period from December 2016 to May 2017. The study was undertaken to evaluatethe toxic effect of chromium on growth, yield and nutritional attributes of BRRI dhan69 and BRRI dhan74. The experiment comprised of two factors, Factor A: two rice cultivars i.e. V1= BRRI dhan69, V2= BRRI dhan74; and six levels of Cr application i.e. T1= 0 mg Cr/kg soil, T2= 12.5 mg Cr/kg soil, T3= 25 mg Cr/kg soil, T4= 50 mg Cr/kg soil, T5= 75 mg Cr/kg soil, T6 =100 mg Cr/kg soil. The experiment was laid out in Completely Randomized Design (CRD) with four replications. Data on different growth parameters, yield attributes, yield and Cr contents were recorded and analyzed. The control treatment attributed the highest values of vegetative growth, yield and yield contributing character, and nutrient content (P, K, S and Na) with V2 variety. Data revealed that the tallest plant was found in T1V1 and shortest plant was found in T6V1 combination also similar trends found in other character. The grain yield of rice ranges from 5.02 t ha-1 to 8.13 t ha-1 while T1V2 produced the highest grains yield and T1V6 produced lowest grains yield. The highest chromium content was found in T6V2. In case of root, there was no value of chromium was found. This might be due to that, applied chromium was transferred from root to grain and straw.
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