ABSTRACT
The cultivation and isolation of Fusarium oxysporum, the causative agent of wilt disease in jute (Corchorus sp.), is crucial for understanding the pathogen and developing effective control measures. Traditional media often allow the growth of a wide range of microorganisms, complicating the isolation of Fusarium oxysporum. This study aimed to develop a semi-selective medium to enhance the isolation and culture of Fusarium oxysporum from infected jute plants, facilitating more efficient and accurate research. Potato dextrose agar (PDA) was supplemented with 50 ppm Streptomycin sulphateand 50 ppm Autostin 50WDG to suppress non-target microbial growth. This advancement provides a valuable tool for plant pathologists and researchers investigating Fusarium oxysporum.
Key words: Semi-selective medium, Fusarium oxysporu, Streptomycin, Autostin, jute, PDA
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