Bangladesh J. Environ. Sci., Vol. 36, 47-50, 2019 @ BAED ISSN 1561-9206
IN VITRO REGENERATION OF SOME SELECTED MUSTARD GENOTYPES (Brassica spp )
N. Sultana1, I. Sultana2 and S. Sultana3
1Dept of Genetics and Plant Breeding, Patuakhali Science and Technology University, Patuakhali
2Agriculture Department, Ghior Govt. College, Manikgonj;
3Lal Teer Seed Ltd. Gazipur
An experiment was conducted to find out in vitro performance of some mustard genotypes (Safal, Sampad and Tori-7). For germination, MS medium was used without any growth regulators. For callus induction, 2, 4-D was used @ 1.5 mg/L concentration and for regeneration, Indole Acetic Acid (IAA) as well as Benzyl Amino Purine (BAP) were used @ 0.5 mg/L and 1.0 mg/L, respectively. The Plant Growth Regulators were used as a single dose combination to ascertain only the genotypic differences. In genotypic sense, Tori-7 and Safal showed better performances compared to Sampad (92.73% and 94% callus induction for Tori-7 and Safal, respectively). On the other hand, genotypes Tori-7 and Sampad ensured better regeneration (72% for Tori-7 and 65% for Sampad) than that of Safal (50%). Therefore; Tori-7 may be termed as the best genotype in terms of both callus induction and regeneration. No statistically significant differences were found for this regarding days required for callus induction but in case of days required for regeneration, the genotypes varied significantly. Both the genotypes Safal (38.98 days) and Sampad (41.79 days) are early regenerating than Tori-7 (45.21 days). All of the genotypes under study have shown their suitability in tissue culture studies with varying abilities.
Key words: In vitro, regeneration, mustard genotypes.