This research work has been done on the creation of desired variants through somaclonal variation by producing callus tissue from the in vitro regeneration of jute (Corchrous olitorius L. Var O-9897). The work was carried out in 2024 at the tissue culture lab of the Cytogenetics Department, Bangladesh Jute Research Institute. Sterile seeds were grown in semisolid agar media without using growth regulators. After 5 to 6 days healthy and sterile plantlets were found (germination above 95%). Cotyledonary shoot tip was used as an explant for in vitro germinated seedlings. After two weeks of cotyledonary shoot tip culture,friable and small size (2.2-4.2 mm) callus growth was found in a medium containing (BAP 2.5 + IAA 0.5), (BAP 0.2 + IAA 1.0); (NAA 0.75+BAP 3.5 + IAA 0.75)and only BAP 2.0with 3% sugar, 1% agar and pH was adjusted at 5.8. Cultures were placed in a growth room with 26 ± 20C under 3000 lumens of fluorescent tubes with 16h photoperiod. Fresh callus was subculture on different concentration and combination of hormone treatments (11 types) for shoot regeneration. MS medium without hormone (control) and with hormone BAP 2.5 + IAA 0.5 and BAP 2.0 gave shoot initiation successfully.

Key words: Tossa jute, callus, hormone, shoot tip and somaclonal variation