Genetic variation among 25 accessions of Corchorus capsularis were determined using SSR primers. In this experiment, 8 primers were used and a total number of 19 loci were amplified by SSR primers. The highest gene diversity value for all primers was 0.659 and the lowest value was 0.218 with an average of 0.439. The values of pair wise comparison of Nei’s genetic distance among jute accessions were computed from combined data ranged from 0 to 0.875. The highest genetic distance value was found among accession no.923 and accession no.1777; accession no.923 and accession no.1778; accession no.927 and accession no.1777; accession no.924 and accession no.937 as well as accession no.931and accession no.937. On the other hand, the lowest genetic distance was found between accession no.1483 and accession no.1489; accession no.925 and accession no.928 as well as accession no.941 and accession no.945. The findings on genetic distance revealed that a high degree of genetic variations were observed among the studied jute accessions. UPGMA dendrogram (based on genetic differences) separated the accessions in three main clusters. So, it is hoped that, in future the present results will help breeders to make selection from the diverse accessions studied and use them as parents for crossing and for producing mapping populations.
Key words: Jute, molecular diversity, DNA fingerprinting
Out of 100 species of Corchorus (Saunders 2001-2006; Benor et al., 2010), only two species viz., C. capsularis L. (white jute) and C. olitorius L. (tossa jute) are cultivated (Ghosh 1983). Closely related cultivars with low genetic variability can’t readily be distinguished by morphological traits like pigmentation pattern, leaf shape, stipule shape, seed coat color and so on (Degani et al., 1998). DNA fingerprinting for cultivar or varietal identification has become an important tool for genetic identification in germplasm management and plant breeding. When planning of DNA fingerprinting, one of the most important decision is the marker system and molecular technique to be used. Various systems and their related techniques are currently available, and those are based on polymerase chain reaction (PCR). PCR based techniques or molecular markers such as Randomly Amplified Polymorphic DNA (RAPDs), Simple Sequence Repeats (SSRs), and Amplified Fragment Length Polymorphisms (AFLPs) are unaffected by environmental factors (Bowditch et al., 1993). SSRs have become a powerful and popular tool for determining unique genetic identity or fingerprint, and establishing genetic relatedness as well as diversity among various crop cultivars. Therefore, the present piece of work is undertaken to develop genetic fingerprints and to estimate genetic relatedness of some white jute (Corchorous capsularis) germplasm using SSR markers.