ABSTRACT

The aim of the experiment was to find out genetic diversity of some jute germplasm collected from different locations of the world that are stored at the gene bank of Bangladesh Jute Research Institute (BJRI). The experiment was conducted at the laboratory of Cytogenetics department of Genetic Resources and Seed Division of BJRI. Genetic variation among 22 jute germplasm including 9 varieties of BJRI were determined using 33 SSR primers. DNA was extracted from seedlings following mini preparation CTAB method with some modifications. Vertical gel electrophoresis system was used with polyacrylamide gel. Among these,   14 primers were polymorphic and amplified 39 loci. The highest gene diversity value among the polymorphic primers was 0.685 and the lowest was 0.177 with an average of 0.507. The highest genetic distance value (1.0) was found between Corchorus capsularis (white jute) and C. olitorius (dark jute/tossa jute) germplasm. Among the C. olitorius germplasm, the highest genetic distance value (0.4) was found between accession no. 1039 and accession no. 1347. Among the C. capsularis germplasm, accession no. 4484 showed the highest genetic distance value (0.214) with varieties CVL-1 and BJRI Deshi Pat Shak-1. UPGMA dendrogram, based on genetic differences separated the germplasm of two different species in two main clusters and the cluster of C. olitorius is subdivided into two sub-clusters. We found no genetic similarity between the two species of jute. Plant breeders can consider the studied germplasm of C. olitorius as more potential for crossing programs as they have good genetic diversity.

Key words: Genetic polymorphism; Genetic variation; Jute; Molecular markers; SSR Markers; UPGMA dendrogram