The freeze-tolerant organisms are gifted with the AFPs/THPs (antifreeze proteins/thermal hysteresis proteins), which have evolved as an adaptation in extreme cold conditions. AFPs have been identified in vertebrates, invertebrates, plants, bacteria, and fungi. In this review, we deal with, diverse applications in the fields of industry, medical and agriculture such as food technology, cell lines and organ preservation, transplantation and transfusion. AFPs have potential applications in agriculture for the production of economically valuable fishes against low temperature. Other proposed applications of AFPs are found in cryosurgery of tumors and as a component of ice-cream to prevent the formation of hard and large ice crystals. Furthermore, the present study focuses the new insights into the development of methodology to allow the production of synthetic AFPs as well as their potential uses.

Key words: Antifreeze protein, cryopreservation, food safety, cryosurgery.