Molecular diversity assessment using ssr primers confirming distinct species of jute germplasm under Corchorus genus

ABSTRACT

The aim of the experiment was to find out genetic diversity of some jute germplasm collected from different locations of the world that are stored at the gene bank of Bangladesh Jute Research Institute (BJRI). The experiment was conducted at the laboratory of Cytogenetics department of Genetic Resources and Seed Division of BJRI. Genetic variation among 22 jute germplasm including 9 varieties of BJRI were determined using 33 SSR primers. DNA was extracted from seedlings following mini preparation CTAB method with some modifications. Vertical gel electrophoresis system was used with polyacrylamide gel. Among these,   14 primers were polymorphic and amplified 39 loci. The highest gene diversity value among the polymorphic primers was 0.685 and the lowest was 0.177 with an average of 0.507. The highest genetic distance value (1.0) was found between Corchorus capsularis (white jute) and C. olitorius (dark jute/tossa jute) germplasm. Among the C. olitorius germplasm, the highest genetic distance value (0.4) was found between accession no. 1039 and accession no. 1347. Among the C. capsularis germplasm, accession no. 4484 showed the highest genetic distance value (0.214) with varieties CVL-1 and BJRI Deshi Pat Shak-1. UPGMA dendrogram, based on genetic differences separated the germplasm of two different species in two main clusters and the cluster of C. olitorius is subdivided into two sub-clusters. We found no genetic similarity between the two species of jute. Plant breeders can consider the studied germplasm of C. olitorius as more potential for crossing programs as they have good genetic diversity.

Key words: Genetic polymorphism; Genetic variation; Jute; Molecular markers; SSR Markers; UPGMA dendrogram

SURVEY ON ARTHROPOD PEST OF CAPSICUM AND DIFFERENT PRACTICES USED BY THE CAPSICUM GROWERS AGAINST INFESTATION IN MAJOR GROWING AREAS OF BANGLADESH

ABSTRACT

The study was conducted at Rajbari, Manikganj, Bogura, Sylhet and Kishoreganj district to study on farmers’ practices for the management of capsicum insect pest in major capsicum growing areas of Bangladesh. Data were collected by the researcher during the period from 15 October to 15 November 2018. Among the respondent the maximum (87.2%) capsicum growers were cultivated capsicum in winter season and 12.8% capsicum growers were cultivated in summer season. The highest around 36.00% have low level infestation whereas only 4.80% were belongs to severe level insect pests’ infestation under the study area. Capsicum growers practices (CGPs) related data were collected at two levels: directly from the sample farmers by administering pre-designed and pre-tested questionnaires. Total 11 capsicum varieties were recorded from the study area and the highest used capsicum varieties California wonder (17.21%), Omax hybrid (15.35%), BARI misti morich -2 (13.84%). BARI misti morich-1 (12.36%), Bell pepper (9.1/0%) and Marcuri rosso (7.23%). Total 10 insect pests of capsicum were recorded from the study area. Among the insect pests capsicum fruit borer and aphid were dominant and observed upto 100% respondent farmer’s field. The capsicum growers practices (CGPs) for capsicum insect pest management were generally three broad types. Type I Non-chemical, bio-pesticide and untreated control practices; Type II was combination of chemicals with other management practices and Type III was combination of non-chemicals management practices comprising bio-pesticide, mechanical, cultural and mulching materials. Most of the respondents of capsicum growers (71.14%) practiced Type II, whereas, Type I was practiced by  23.08% and Type III was practiced by only 5.77% of respondents of capsicum growers of the respective districts. The BCR was maximum in CGP 8, CGP 9, CGP11, CGP 12 and CGP 10 (3.12-2.90) while it was minimum (2.06-2.15) in CGP 7 and CGP 15. Application of integrated pest control measures were more effective compared to single control measure for capsicum cultivation regarding insect pest management.

Key words: Capsicum, arthropod pests, infestation, practices.

General evaluation of two success stories of M. A. MIYAN on IUBAT and KBAD

ABSTRACT

Prof. Dr. M.A. Miyan established IUBAT with wide success where he developed two concepts regarding IUBAT and KBAD and published those materials in two reports and referred in the references. On the basis/guideline of the reports, concepts, mission, vision and philosophy of IUBAT and KBAD are developed for future generations. Fig. 1. showed the KBAD’s summary as mission and vision.

Key words: P IUBAT, KBAD, concepts, mission, vision, philosophy.

BACKGROUND, MISSION, VISION AND OPERATIONAL PHILOSOPHY OF IUBAT

IUBAT-International University of Business Agriculture and Technology is the first Nongovernment university in Bangladesh. Prof. Dr. M. Alimullah Miyan was the founder of IUBAT as well as the pioneer regarding the building/development of private university in Bangladesh.In 1980s there were only six universities like Dhaka University, Rajshahi University, Chittagong University, Jahangirnagar University, BAU and BUET where admission capacity covered only 10000-12000 students although there were several lakh good students (better SSC and HSC results) failed to proceed for higher studies. Then thousands of students moved to India and that was the great business for many universities of India. It was really bad days regarding higher education in Bangladesh. Prof. Dr. Alimullah Miyan, Director of the Institute of Business Administration (IBA), Dhaka University with wide depth of national and international latest knowledge, observed the university education crisis of the children came forward with private university concepts. With World Bank sponsor, he visited Kansas State University (KSU), Manhattan, Kansas, USA for 3 weeks where he faced a lot of discussions with 40 Academicians regarding role of universities of USA relevant to society and tried to discover the application of such role of university to the society in Bangladesh. Dr. M.A. Miyan is basically rich is Business Administration and KSU is rich in Agriculture. Moreover, for food security, agriculture is important for Bangladesh. So, Dr Miyan finally took two major issues covering business and agriculture in his concepts, mission and vision and then developed a proposal on “Proposal for establishing a university of business, agriculture and technology at Dhaka, Bangladesh with academic collaboration and recognition of Kansas State University” on April 3, 1989 under his guideline on “Non-government University movement in Bangladesh”. The proposal widely and excellently covered under proper headlines like Introduction, Background, Objectives, Scope of the university, Organization, Resources, Conclusions and a Structure of the University (model). His presentation and discussions with KSU Academician’s were widely fruitful and successful and finally KSU authority came forward to help him in all aspects of academic matters and gave him a letter by Verman C. Harson on April 27, 1989 covering “letter of intent to participate in a new university in Bangladesh”. That was the great success of Dr. Miyan to develop a University of Business Agriculture (UBA) in Bangladesh.

Sources, problems, health hazards and management of dust contaminations at Dhaka metropolitan city

ABSTRACT

A research on evaluation of dust/clay colloids (diameter size 0.002/0.0002mm) i.e. PM 2.5 (less tha 2.5 m size diameter) contaminations to air were investigated where major sources of dusts/clays from soils of various categories were reported. Such dust/clay colloids contaminations occurred by CO2, CO, SO2, SOx, N2O, NOx, heavy metals like Pb, As, Cd, Cr, Ni, Hg, Co, etc move in air although soil has 16-24 heavy metals contaminants with various concentrations. Again, Coal-fired power plants carry lots of heavy metals like Zn, Se, Cd, Ni, Pb, Hg, Cr, V, Br, As, Ur, Ti, CO2, CH4, NOx although most N2O, N, NOx etc. come from N-fertilizers. Such minor to major polluted/contaminated dust/dust colloids in air move/enter the human body as toxic/contaminated air and acts as slow poison and producing minor to major diseases including cancer. Here dusts related diseases (more than 20) are listed. Again, dust sources, contaminations and health hazards were also compared between developed countries and Bangladesh. Air is not fresh one at Dhaka i.e. air at Dhaka city means dust/dust colloid contaminated air. The satellite observation of PM 2.5 or more may or may not be severely contaminated until lab. analysis and detections covering of various heavy metals, widely used pesticide residues/contaminants and other pollutants in air although black dust/fume dusts carry huge contaminants. Finally, wide suggestions and recommendation are applied against dust contaminations in air. The entire study, observations, measurement guidelines are well analysed through five tables (Tables 1-5) and five figures (Figs. 1-5) and they are summarized and presented as schematic model in Fig. 1.

Key words: Dusts, dust colloids, clay colloids, contaminations, problems, health hazards, sources of dust contaminations, comparisons, heavy metals, suggestion/guidelines.

এদেশে পরিবেশ বিজ্ঞান শিক্ষা ও গবেষণা শুরুর পূর্বে (১৯৮৯-৯১) বিজ্ঞানী সাত্তারের পরিবেশ বিজ্ঞান বিষয়ে ১১৩টি প্রকাশনা পরিচিতি

প্রফেসর ড. এম.এ. সাত্তার, ডিন, জীব বিজ্ঞান অনুষদ, বঙ্গবন্দু শেখ মুজিবুর রহমান বিজ্ঞান ও প্রযুক্তি বিশ্ববিদ্যালয়, গোপালগঞ্জে ২০১৬ সাল থেকে কর্মরত যেখানে বিভাগে তিনি একমাত্র শিক্ষক হিসেবে ২০১৬/১৭ সালে পরিবেশ বিজ্ঞান ও ডিজাষ্টার ম্যানেজমেন্ট বিভাগ প্রতিষ্ঠা করেন। তার আগে তিনি বাকৃবিতে ৪২ বৎসর শিক্ষকতা (১৯৭৩-২০১৪) করে কৃষি অনুষদের ডিন হিসেবে অবসরে যান। তার ১৯৯১-২০০২ সালে চেষ্টায় বাকৃবিতে ২০০২ সালে পরিবেশ বিজ্ঞান বিভাগ প্রতিষ্ঠা লাভ করে। তিনি এদেশে পরিবেশ বিজ্ঞান শিক্ষা ও গবেষণার অগ্রগতিতে আজীবন প্রত্যক্ষ জড়িত। মাটিতে ইউরিয়ার জীবন চক্র, ইউরিয়ার জৈব পদার্থ ও এনপির ক্ষয়/ঘাটতি তথা ইউরিয়া প্রয়োগের পাশাপাশি জৈব পদার্থ প্রয়োগ মৃত্তিকা পরিবেশ বিষয়ে মৌলিক আবিস্কারের মাধ্যমে ১৯৭২ সাল থেকেই এদেশে পরিবেশ বিজ্ঞান জগতে আত্মপ্রকাশ করেন। তিনি ৯.০ বৎসর (১৯৭৬-৮৪) ফিনল্যান্ডে পরিবেশ বিজ্ঞান শিক্ষা ও গবেষণায় ৪টি পিএইচডি সমমান ডিগ্রী অর্জন সহ অসংখ্য আন্তর্জাতিক প্রকাশনার মাধ্যমে বিশ্বে আন্তর্জাতিক খ্যাতি সম্পন্ন বিজ্ঞানীদের কাতারে চলে আসেন।

Vegetative performances of BARI hybrid maize-7 as influenced by nitrogen doses and irrigation frequencies

ABSTRACT

A field study was conducted to assess the interaction effects of irrigation and nitrogen rates on hybrid maize yield. The research was conducted during December 2013 to April 2014 at the farmer field Tabaria area of Natore District in Bangladesh. The experiment consists of (i) two irrigation treatments viz. no irrigation and three irrigation and (ii) two nitrogen levels viz. no nitrogen use and 230 kg Nha-1.The experiment was laid out in a split plot design with three replications having 12 unit plots with 20 m2 sized. The interaction effect of irrigations and nitrogen levels significantly influenced the studied vegetative performances of BARI hybrid maize-7, where the growth attributes increased with increasing irrigation and nitrogen levels in all cases.

Key words: Nitrogen, irrigation, growth, maize.

Introduction The crop maize is gaining popularity in the country very quickly due to its high yield potential. Maize acreage and production have an increasing tendency with the introduction of hybrid since 1993 (Karim      et al., 2010). The population growth in Bangladesh puts great pressure on the country’s food production. As regard food, cereal is still staple one for Bangladeshi people. In terms of human consumption maize also occupies the third position after rice and wheat in the country (HIES, 2005).The country is importing a huge amount of maize with the increasing demand from poultry and other feed industries. Therefore, maize has gained an increasingly important attention by the government. Now days, there are many organizations who are working for increasing maize production in Bangladesh. Maize production increased significantly during the last decade and still there is a large demand-supply gap. Local production cannot meet the demand and hence imports fill that gap. The growth of maize production did not spread all over the country though there are potential and still now is mainly concentrated in the north and north-western part of Bangladesh. However, Maize has high yield potential and responds well to different management practices. Among various management practices, irrigation and nitrogen play a significant role in realizing the maximum potential of the crop. Irrigation scheduling is the technology for applying the proper amount of water at the right time. Reasons for using irrigation scheduling are to reduce water applications, energy consumption and deep percolation of water below the crop root zone. Water is further required to provide constant turgor pressure that supports the plant and facilitates cell enlargement after cell division has been initiated. Hence, plant growth and survival depend on adequate water availability. Hence, the present study was therefore, undertaken to determine the optimum level of irrigation schedules and nitrogen rates for enhanced growth of maize in Bangladesh.